Fluid and DNA Sampler

Understanding the linkages among microbes that thrive beneath the seafloor and in vent fluids associated with active submarine volcanoes is one of the most profound areas of marine research. New discoveries in this area require in situ measurements of fluid chemistry and temperature, and the ability to take co-registered filtered samples of microbial DNA for eventual shore-based analyses that allow identification of the organisms and information about their novel metabolisms.

The RAS (Remote Access Sampler) allows time-series temperature measurement in real-time and in situ sampling of hydrothermal vent fluids, while the PPS (Particulate DNA Sampler) allows collection, filtering, and preservation of microbial DNA. This coupled system, developed by D. Butterfield at NOAA-PMEL, was installed from 2014-2017 in the International District Hydrothermal Vent Field at the El Gordo diffuse flow site in the International District Hydrothermal Field in Axial Caldera. In 2017, the RAS and microbial sampler were placed in a junction box frame, along with the mass spectrometer, and installed at the Tiny Village site.The intake line to the instruments is placed inside a “vent cap” along with an intake for the mass spectrometer to obtain co-registered temperature, volatile vent fluid chemistry, and microbial DNA analyses at this site. Controlled from shore, or by a mission plan, a pump sucks in hydrothermal fluid into sterile bags (and also onto filters for collection of DNA in a similar system above the RAS). A nearby digital still camera provides an overview of the vent and documentation of changes in vent deposit evolution and macrofaunal communities over time. Because the instrument is cabled, which allows two-way communication and power, the sample bottles can be triggered from shore in response to volcanic or tectonic events, or set to trigger at specified intervals.

During annual Cabled Array maintenance cruises, this coupled mooring is recovered and collected material analyzed to provide chemical characterization of the hydrothermal fluid samples. The OOI Core Instrument Analytical Results data repository contains processed analytical data and metadata for a subset of Cabled Array instruments that collect fluid or particulate samples that are brought back to shore and analyzed in a laboratory. Numerical and other processed analytical results are generally provided in spreadsheet form. Associated metadata files including analytical protocols, sampling and recovery notes, and sample log files are presented in .pdf format. As-deployed images of instruments are provided in .jpg format, with image metadata summarized in spreadsheet-based catalogs.

The RASFL analytical results can be found here: International District Vent Field RASFL Data

During ROPOS Dive R1729, a digital-still camera (left), a mass spectrometer (middle) and a fluid- and microbial-DNA sampler (right) were installed in the International District Hydrothermal Field at the vent called El Gordo. A titanium “hat” rests on top of the structure in a tubeworm and limpet patch. Inside the “hat” are temperature probes and intake nozzles for the fluid and DNA sampler. Photo credit: NSF-OOI/UW/CSSF; Dive R1729; V14. Creative Commons License

Share this page

Share on facebook
Share on google
Share on twitter
Share on linkedin
Share on print
Share on email
Close Panel